Engineering pore-forming proteins as tools for the delivery of nano-particles
This project started in 2011 along with Dr Carlos Rosado. Our current focus is to understand how Perforin undergoes conformational change upon calcium binding to trigger pore formation. We recently solved several crystal structures of the membrane-binding domain of Perforin homologues in both calcium free and calcium bound forms. These structures bring us new insight into the calcium dependent membrane binding mechanism of Perforin.
Structural studies on Clostridium perfringens conjugation system
In collaboration with Prof Julian Rood (Microbiology Department), this project seeks to understand the mechanism by which antibiotic resistance is transferred via the conjugative apparatus in Gram-negative bacteria. We recently solved the crystal structure of TcpC (Porter et al. 2012) and we ultimately aim to solve the structure of the components of the entire conjugation apparatus.
Both projects combine biochemistry and molecular biology techniques with structural characterization methods including X-ray crystallography and Electron microscopy.
Yoga, Y.M.K., Traore, D.A., Sidiqi, M., Szeto, C., Pendini, N.R., Barker, A., Leedman, P., Wilce, J.A., Wilce, M.C., 2012, Contribution of the first K-homology domain of poly(C)-binding protein 1 to its affinity and specificity for C-rich oligonucleotides, Nucleic Acids Research [P], vol E-pub, Oxford University Press, UK, pp. 1-14.
Peddie, V., Butcher, R.J., Robinson, W.T., Wilce, M.C., Traore, D.A.K., Abell, A.D., 2012, Synthesis and Conformation of Fluorinated beta-Peptidic Compounds, Chemistry-A European Journal [P], vol E-pub, Wiley - V C H Verlag GmbH & Co KGaA, Germany, pp. 1-9.
Lassaux, P., Traore, D.A.K., Loisel, E., Docquier, J.-., Sohier, J.S., Laurent, C., Bebrone, C., Frere, J.-., Ferrer, J.-., Galleni, M., 2011, Biochemical and Structural Characterization of the Subclass B1 Metallo--Lactamase VIM-4, Antimicrobial Agents And Chemotherapy [P], vol 55, issue 3, American Society for Microbiology, USA, pp. 1248-1255.
Don Paul, C., Traore, D., Byres, E., Rossjohn, J., Devenish, R., Kiss, C., Bradbury, A., Wilce, M., Prescott, M., 2011, Expression, purification, crystallization and preliminary X-ray analysis of eCGP123, an extremely stable monomeric green fluorescent protein with reversible photoswitching properties, Acta Crystallographica Section F: Structural Biology and Crystallization Communications [P], vol 67, issue 10, Wiley-Blackwell Publishing, Inc., USA, pp. 1266-1268.
Yoga, Y.M.K., Traore, D.A., Wilce, J.A., Wilce, M.C., 2011, Mutation and crystallization of the first KH domain of human polycytosine-binding protein 1 (PCBP1) in complex with DNA, Acta Crystallographica Section F: Structural Biology and Crystallization Communications [P], vol 67, issue Pt 10, Wiley-Blackwell Publishing, Inc., USA, pp. 1257-1261.
Traore, D.A.K., Ghazouani, A.E., Jacquamet, L., Borel, F., Ferrer, J.-., Lascoux, D., Ravanat, J.-., Jaquinod, M., Blondin, G., Caux-Thang, C., Duarte, V., Latour, J.-., 2009, Structural and functional characterization of 2-oxo-histidine in oxidized PerR protein, Nature Chemical Biology [P], vol 5, issue 1, Nature Publishing Group, UK, pp. 53-59.
Iwema, T., Picciocchi, A., Traore, D.A.K., Ferrer, J., Chauvat, F., Jacquamet, L., 2009, Structural basis for delivery of the intact [Fe2S2] cluster by monothiol glutaredoxin, Biochemistry [P], vol 48, issue 26, American Chemical Society, US, pp. 6041-6043.
Jacquamet, L., Traore, D.A.K., Ferrer, J.-., Proux, O., Testemale, D., Hazemann, J.-., Nazarenko, E., El Ghazouani, A., Caux-Thang, C., Duarte, V., Latour, J.-., 2009, Structural characterization of the active form of PerR: Insights into the metal-induced activation of PerR and fur proteins for DNA binding, Molecular Microbiology [P], vol 73, issue 1, Wiley-Blackwell Publishing Ltd, UK, pp. 20-31.
Traore, D.A.K., El Ghazouani, A., Ilango, S., Dupuy, J., Jacquamet, L., Ferrer, J., Caux-Thang, C., Duarte, V., Latour, J., 2006, Crystal structure of the apo-PerR-Zn protein from Bacillus subtilis, Molecular Microbiology [P], vol 61, issue 5, Wiley-Blackwell Publishing Ltd, UK, pp. 1211-1219.